![]() There is currently no content classified with this term. Ridiculously easy experiment planning with range of concentrations or dilution factor. The serial dilution process is also used to make the standard curves needed for many quantitative procedures. Serial dilution calculator for dose response EC50, IC50 curves. For example, a ten-fold serial dilution could result in the following concentrations: 1 M, 0.1 M, 0.01 M, 0.001 M, and so on. Typically, the dilution factor remains constant for each dilution, resulting in an exponential decrease in concentration. Serial dilutions are made when a sample is too concentrated for a particular procedure such as a colorimetric assay. Serial dilutions involve diluting a stock or standard solution multiple times in a row. This second step produces a 100x dilution. The 10x dilution can then be diluted by a factor of 10 again by mixing it with 9 more parts of the diluent. As a result, the diluted sub-samples can be used for a variety of procedures without consuming the bulk of the original sample.Ī 10x dilution is obtained by mixing 1 part of a sample with 9 parts of a diluent so that the new solution is 10 times (10x) less concentrated than the original solution. A number of spread plates is needed because the exact number of live bacteria in the sample is usually unknown. 10 -2 to 10 -8 ) is normally performed on the sample culture and spread plates created from the dilutions. Method Using aseptic technique, dispense 9 mL of media into each of ten test tubes with sterile automatic dispenser or sterile 10 mL pipettes. Often 10% or less of the original sample is used in the first step of a dilution series. Figure 1: Serial dilution series and plating. The uniform nature of the process makes it possible to back-calculate characteristics of the original solution based on procedures performed the diluted samples. ![]() ![]() The process of reducing the concentration * of a solution by mixing small volume *s of the original solution with water (or other solvent *) over a number of uniform steps.
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